# JXB Fig2 — 蛋白-转录本差异（GPT/Gemini通用Prompt）

A two-panel scientific figure (A–B), white background, vector style, Arial font.

**Title:** Transcript–protein divergence between dwarf cultivars

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## Panel A — Volcano plot（火山图）
**Type:** Volcano plot comparing W5 vs W6 proteome at mid-stage.

**Data:**
- Total proteins: 7,550 identified (early + mid)
- Mid-stage DEPs (W5 vs W6): 2,851 differentially abundant proteins (fold-change > 1.5, FDR q < 0.05)

**Visual:**
- X-axis: log₂(fold-change) W5/W6
- Y-axis: −log₁₀(FDR q-value)
- Significantly upregulated in W5: red dots
- Significantly downregulated in W5: blue dots
- Not significant: gray dots
- Vertical dashed lines at FC = 1.5 and −1.5
- Horizontal dashed line at FDR = 0.05

**Subtitle:** "Differentially abundant proteins (DEPs), W5 vs W6, mid-stage"

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## Panel B — Comparison bar chart（对比柱状图）
**Type:** Side-by-side bar chart comparing DEPs vs DEGs.

**Data:**
- Mid-stage W5 vs W6:
  - Differentially abundant proteins (DEPs): 2,851
  - Differentially expressed genes (DEGs): 2,183 (418 up + 1,765 down)

**Visual:**
- Two bars: left = "DEPs" (orange #E67E22), right = "DEGs" (steel blue #2E86C1)
- Y-axis labeled "Number of features"
- Show "1.3×" bracket above with annotation: "Transcript–protein divergence"

**Subtitle:** "Mid-stage differential abundance, transcript vs protein"

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## Panel layout
- Layout: 1 row × 2 columns (A | B)
- Panel labels: **A**, **B** in bold at top-left
- All axes and legends ≥10pt, no gridlines, white background